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Antibiotic Discovery
Learned and executed the process of antibiotic discovery
1. Bacterial Culture
Grow Lysobactor bacteria, which produces antibiotic compounds
2. Liquid-Liquid Extraction
Crude extraction from bacterial culture of organic molecules in ethanol layer from aqueous layer
3. Thin Layer Chromatography
Determine optimum solvent for column chromatgarphy
4. Column Chromatography
Separation of organic molecules based on differences in polarity
5. Antibiotic Assay Flow Chart
Determine which (if any) isolated organic compounds are antibiotic
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6. High-performance Liquid Chromatography
Pure extraction of antibiotic organic compound
7. Identification of Antibiotic Compound
Using HNMR and Mass spectrometry
HNMR Spectra
Mass Spectrometry Spectra
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